There are 3 users that will access the PI Server from their separate logins, that I have already created in PI SMT and mapped as well. As of now I have tested that any of the three pi users can login to the server simultaneously from all three systems remotely. I wanted to inquire is there any way to restrict a PI User to login to the PI Server from only 1 remote system at a time?
It is not possible as the PI Server will look at the Windows credentials (AD) and allow if it has a mapping and give it its respective access. You can blacklist an IP address or a set of IP's but what you appear to want to do (allow a domain user from logging in on only 1 machine at a time) is not possible.
What is the reason behind wanting to do this? We can possibly find an alternative option.